Chihuly's monumental glass sculptures have been exhibited at several other famous locations across the United States, including the Atlanta Botanical Gardens.
Tourists said they were excited to see the colorful display come to Asheville.
"I think it's very unique -- the blown glass and the steel, the very detailed textures of it, and you can even come up with your own idea of what they might be or what he interpreted," Stephanie Corey, of Charleston, South Carolina, said.
Biltmore will also host an evening experience that will offer views of the sculptures after dark.
Daytime admission to the exhibit is included in the general admission ticket price, but a separate ticket is needed for the evening show.
The exhibit runs from May 17 - Oct. 7.